aroE [2019-01-23 19:37:45]

3-phosphoshikimate 1-carboxyvinyltransferase

Locus: BSU22600

Isoelectric point: 6.34

Molecular weight: 45.08 kDa

Protein length: 428 aa

Gene length: 1284 bp

Function: biosynthesis of aromatic amino acids

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Essential: yes

E.C.: 2.5.1.19

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids
• Groups of genes → Essential genes

Gene

Coordinates: 2,367,954 → 2,369,240

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Phosphoenolpyruvate 3-phosphoshikimate = phosphate 5-O-(1-carboxyvinyl)-3-phosphoshikimate (according to Swiss-Prot)

Structure

• 3RMT (from B. halodurans, 68% identity, 87% similarity)

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE
  Description: PubMed

  Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

• Genes: hisC-tyrA-aroE
  Description: PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• BKE22600 (ΔaroE::erm trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CTTATCTCGTTTCATTTTTT, downstream forward: _UP4_TGAAGTTTTACTTCAGGATT

Expression vectors

• for expression in B. subtilis, in pBQ200: piGEM2, available in Fabian Commichau's lab

References

Reviews

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Original publications

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